Hoxworth

Publications and Patents

Publications

1. Slichter SJ, Dumont LJ, Cancelas JA, Jones M, Gernsheimer TB, Szczepiorkowski ZM, Dunbar NM, Prakash G, Medlin S, Rugg N, Kinne B, Macdonald VW, Housler G, Valiyaveettil M, Hmel P, Ransom JH. Safety and efficacy of cryopreserved platelets in bleeding patients with thrombocytopenia. Transfusion. 2018 Sep;58(9):2129-2138. doi: 10.1111/trf.14780. PMID 30204953

2. Zhang C, D'Alessandro A, Wellendorf AM, Mohmoud F, Serrano-Lopez J, Perentesis J, Komurov K, Alexe G, Stegmaier K, Whitsett J, Grimes HL, Cancelas JA. KLF5 controls glutathione metabolism to suppress p190-BCR-ABL+ B-cell lymphoblastic leukemia. Oncotarget Jul 3, 2018;9(51)29665-79. doi: 10.18632/oncotarget.25667 PMID 30038712. PMC6049869

3. Mock DM, Nalbant D, Kyosseva SV, Schmidt RL, An G, Matthews NI, Vlaar APJ, van Bruggen R, de Korte D, Strauss RG, Cancelas JA, Franco RS, Veng-Pedersen P, Widness JA. Development, validation, and potential applications of biotinylated red blood cells for posttransfusion kinetics and other physiological studies: evidenced-based analysis and recommendations. Transfusion Aug 2018;58(8):2068-81. Epub May 16 2018. doi: 10.1111/trf.14647. Review. PMID 29770455

4.Roy S, Rai P, Eiymo Mwa Mpollo MS, Chang KH, Rizvi T, Shanmukhappa SK, VandenHeuvel K, Aronow B, Inagami T, Cancelas JA, Malik P. Angiotensin receptor signaling in sickle cell anemia has a reno-protective effect on urine concentrating ability but results in sickle glomerulopathy. Am J Hematol Jul 2018;93(7):E177-E181. (Letter) Epub 2018 Apr 20. doi: 10.1002/ajh.25118. PMID 29675906. NIHMS961332. PMC6037553 [Available on 2019-07-01]

5.Fang J, Muto T, Kleppe M, Bolanos LC, Hueneman KM, Walker CS, Sampson L, Wellendorf AM, Chetal K, Choi K, Salomonis N, Choi Y, Zheng Y, Cancelas JA, Levine RL, Starczynowski DT. TRAF6 mediates basal activation of NF-κB necessary for hematopoietic stem cell homeostasis. Cell Rep 2018 Jan 30;22(5):1250-1262. doi: 10.1016/j.celrep.2018.01.013. PMID 29386112 NIHMS966903. PMC5971064

6. Serrano-Lopez J, Nattamai K, Pease NA, Shephard MS, Wellendorf AM, Sertorio M, Smith EA, Geiger H, Wells SI, Cancelas JA, Privette Vinnedge LM. Loss of DEK induces radioresistance of murine restricted hematopoietic progenitors. Exp Hematol 2018 Mar;59:40-50.e3. doi: 10.1016/j.exphem.2017.12.009. Epub Dec 27 2017. PMID 29288703 PMC5844846 [Available on 2019-03-01]

7. Lee JM, Govindarajah V, Goddard B, Hinge A, Muench DE, Filippi MD, Aronow B, Cancelas JA, Salomonis N, Grimes HL, Reynaud D. Obesity alters the long-term fitness of the hematopoietic stem cell compartment through modulation of Gfi1 expression. J Exp Med. 2018 Feb 5;215(2):627-644. doi: 10.1084/jem.20170690. Epub 2017 Dec 27. PMID 29282250, PMC5789409

8. Wang J, Hayashi Y, Yokota A, Xu Z, Zhang Y, Huang R, Yan X, Liu H, Ma L, Azam M, Bridges JP, Cancelas JA, Kalfa TA, An X, Xiao Z, Huang G. Expansion of EPOR-negative macrophages besides erythroblasts by elevated EPOR signaling in erythrocytosis mouse models. Haematologica 2018;103(1):69-79. Epub 2017 Oct 19. pii: haematol.2017.172775. doi: 10.3324/haematol.2017. 172775. PMID 29051279, PMC5777189

9. Nalbant D, Cancelas JA, Mock DM, Kyosseva SV, Schmidt RL, Cress GA, Zimmerman MB, Strauss RG, Widness JA. In premature infants there is no decrease in 24-hour posttransfusion allogeneic red blood cell recovery after 42 days of storage. Transfusion 2018;58:352-8. Epub 2017 Nov 29. doi: 10.1111/trf.14396 PMID 29193118 PMC5803343

10. Noel JG, Ramser BJ, Cancelas JA, McCormack FX, Gardner JC. Thermal injury of the skin induces G-CSF dependent attenuation of EPO-mediated STAT signaling and erythroid differentiation arrest in mice. Exp Hematol 2017;56:16-30. Epub 2017 Aug 31. doi: 10.1016/j.exphem.2017.08.005. PMID 28867537

11. Worsham DN, Reems J-A, Szczepiorkowski ZM, McKenna DH, Leemhuis T, Mathew AJ, Cancelas JA, on behalf of the Biomedical Excellence for Safer Transfusion (BEST) Collaborative Group. Clinical methods of cryopreservation for donor lymphocyte infusions vary in their ability to preserve functional T-cell subpopulations. Transfusion 2017 Jun;57(6):1555-1565. DOI: 10.1111/trf.14112 PMID 28453219

12. Liu S, de Castro LF Jin P, Civini S, Ren J, Reems J-A, Cancelas JA, Nayak R, Shaw G, O'Brien T, McKenna Jr DH, Armant M, Silberstein L, Gee AP, Hei DJ, Hematti P, Kuznetsov SA, Robey PG, Stroncek DF. Manufacturing differences affect human bone marrow stromal cell characteristics and function: comparison of production methods and products from multiple centers. Sci Rep 2017 Apr 27;7:46731. doi: 10.1038/srep46731 PMID 28447618 PMC5406832

13. Cancelas JA, Slichter SJ, Rugg N, Pratt PG, Nestheide S, Corson J, Pellham E, Huntington M, Goodrich RP. Red blood cells derived from whole blood treated with riboflavin and ultraviolet light maintain adequate survival in vivo after 21 days of storage. Transfusion 2017 May;57(5):1218-25. epub 31 MAR 2017 | DOI: 10.1111/trf.14084. PMID 28369971.

14. Daily K, Ho Sui SJ, Schriml LM, Dexheimer PJ, Salomonis N, Schroll R, Bush S, Keddache M, Mayhew C, Lotia S, Perumal TM, Dang K, Pantano L, Pico AR, Grassman E, Nordling D, Hide W, Hatzopoulos AK, Malik P, Cancelas JA, Lutzko C, Aronow BJ, Omberg L. Molecular, phenotypic, and sample-associated data to describe pluripotent stem cell lines and derivatives. Sci Data 2017 Mar 28;4:170030. doi: 10.1038/sdata.2017.30. PMID 28350385 PMC5369318

15. Kesarwani M, Kincaid Z, Gomaa A, Huber E, Rohrabaugh S, Siddiqui Z, Bouso MF, Latif T, Xu M, Kumarov K, Mulloy JC, Cancelas JA, Grimes HL, Azam M. Targeting c-FOS and DUSP1 abrogates intrinsic resistance to tyrosine-kinase inhibitor therapy in BCR-ABL-induced leukemia. Nat Med 2017 Apr;23(4):472-82. Epub Mar 20, 2017. doi: 10.1038/nm.4310. PMID 28319094 PMC5424814.

16. Schmidt RL, Mock DM, Franco RS, Cohen RM, North AK, Cancelas JA, Geisen C, Strauss RG, Vlaar AP, Nalbant D, Widness JA. Antibodies to biotinylated red blood cells in adults and infants: improved detection, partial characterization, and dependence on RBC-biotin dose. Transfusion 2017;57(6):1488-96. Epub Mar 5, 2017. doi: 10.1111/trf.14075. PMID 28261808

17. Choi K, Komurov K, Fletcher J, Jousma E, Cancelas JA, Wu J, Ratner N. An inflammatory gene signature distinguishes neurofibroma Schwann cells and macrophages from cells in the normal peripheral nervous system. Sci Rep 2017 Mar 3;7:43315. doi: 10.1038/srep43315. PMID 28256556 PMC5335359

18. Guidi N, Sacma M, Standker L, Soller K, Marka G, Weiss J, Kirchhoff F, Weil T, Cancelas JA, Florian MC, Geiger H. Osteopontin attenuates aging-associated phenotypes of hematopoietic stem cells. EMBO J 2017 May 15;36(7):840-53. Epub 2017 Mar 2. pii: e201694969. doi: 10.15252/embj.201694969. PMID 28254837 PMC5376966 (Erratum 2017 May 15;36(10):1463. doi: 10.15252/embj.201796968. PMID28507084 PMC5430211)

19. Cancelas JA, Gottschall JL, Rugg N, Graminske S, Schott MA, North A, Huang N, Mufti N, Rico S, Corash L. Red blood cell concentrates treated with the amustaline (S-303) pathogen reduction system and stored for 35 days retain post-transfusion viability: results of a two-centre study. Vox Sang 2017 Apr;112(3)210-8. Epub Feb 21. doi: 10.1111/vox.12500. PMID 28220519 Erratum: Vox Sang 2017 Jul;112(5):495 doi: 10.111/vox.12535 PMID 28727151

20. Cancelas JA, Rugg N, Nestheide S, Hill SE, Gorgas GD, Emanuele RM, McKenzie DS. The purified vepoloxamer, prevents hemolysis in 42-day stored, DEHP/PVC-free RBC units. Blood Transfus 2017 Mar;15(2):165-71. http://www.bloodtransfusion.it/articolo.aspx?idart=003030&idriv=000119 PMID 28263175 PMC5336339

21. Cancelas JA, Scott EP, Bill JR. Continuous CD34+ cell collection by a new device is safe and more efficient than by a standard collection procedure: results of a two-center, crossover, randomized trial. Transfusion 2016 Nov;56(11):2824-32. Epub Sep 2. doi: 10.1111/trf.13769. PMID 27589225

22. Salomonis N, Dexheimer PJ, Omberg L, Schroll R, Bush S, Huo J, Schriml L, Sui SH, Keddache M, Mayhew C, Shanmukhappa SK, Wells J, Daily K, Hubler S, Wang Y, Zambidis E, Margolin A, Hide W, Hatzopoulos AK, Malik P, Cancelas JA, Aronow B, Lutzko C. Integrated genomic analysis of diverse induced pluripotent stem cell from the Progenitor Cell Biology Consortium. Stem Cell Rep. 2016 Jul 12;7(1):110-25. Epub Jun 9 2016. PMID 27293150 PMC4944587

23. Wu J, Keng V, Patmore DM, Kendall JJ, Patel AV, Jousma E, Jessen WJ, Choi K, Tschida BR, Silverstein KA, Fan D, Schwartz EB, Fuchs JR, Zou Y, Kim MO, Dombi E, Levy DE, Huang G, Cancelas JA, Stemmer-Rachamimov AO, Spinner RJ, Largaespada DA, Ratner N. Insertional mutagenesis identifies a STAT3/Arid1b/β-catenin pathway driving neurofibroma initiation. Cell Rep 2016 Mar 1;14(8):1979-90. Epub 2016 Feb 18. PMID26904939 NIHMS757281 PMC4782770

24. Gonzalez-Nieto D, Chang K-H, Fasciani I, Nayak R, Fernadez-Garcia L, Barrio LC, Cancelas JA. Connexins: Intercellular Signal Transmitters in Lymphohematopoietic Tissues. Chapter 2 in KW Jeon (Ed), Int Rev Cell Mol Biol 2015;318:27-62. Epub Jul 22 2015. PMID 26315883

25. Nayak RC, Trump L, Aronow BJ, Myers K, Mehta P, Kalfa T, Wellendorf AM, Valencia CA, Paddison PJ, Horwitz MS, Grimes HL, Lutzko C, Cancelas JA. Pathogenesis of ELANE-mutant severe neutropenia revealed by induced pluripotent stem cells. J Clin Invest 2015 Aug 3;125(8):3103-16. Epub 2015 Jul 20. pii: 80924. doi: 10.1172/JCI80924. PMID 26193632 PMC4563755.

26. Cancelas JA, Padmanabhan A, Le T, Ambruso DR, Rugg N, Worsham DN, Pinkard SL, Graminske S, Buck J, Goldberg J, Bill J. Spectra Optia granulocyte apheresis collections result in higher collection efficiency of viable, functional neutrophils in a randomized, crossover, multicenter trial. Transfusion, 2015 Apr;55(4):748-55. Epub Nov 10 2014. PMID 25382805

27. Dumont LJ*, Cancelas JA*, Maes LA, Rugg N, Whitley P, Herschel L, Siegel AH, Szczepiorkowski ZM, Hess JR, Zia M. Overnight, room-temperature hold of whole blood followed by 42-day storage of RBCs in additive solution-7. * co-first authors. Transfusion 2015 Mar;55(3):485-90. Epub Sep 19, 2014. doi: 10.1111/trf.12868. PMID 25234026

28. Cancelas JA, Dumont LJ, Maes LA, Rugg N, Herschel L, Whitley PH, Szczepiorkowski ZM, Siegel AH, Hess JR, Zia M. Additive solution-7 (AS-7) reduces the red blood cell cold-storage lesion. Transfusion 2015 Mar;55(3):491-8. Epub Sep 19, 2014. PMID 25233911

29. Dumont LJ*, Cancelas JA*, Maes LA, Rugg N, Whitley P, Herschel L, Siegel AH, Szczepiorkowski ZM, Hess JR, Zia M. The bioequivalence of frozen plasma prepared from whole blood held overnight at room temperature compared to fresh-frozen plasma prepared within eight hours of collection. *co-first authors. Transfusion 2015 Mar;55(3):476-84. Epub Sep 19, 2014. PMID 25233805

30. Chang KH, Nayak R, Roy S, Perumbeti A, Wellendorf AM, Bezold KY, Pirman M, Hill SE, Starnes J, Loberg A, Zhou X, Inagami T, Zheng Y, Malik P, Cancelas JA. Vasculopathy associated hyperangiotensinemia mobilizes hematopoietic stem cells/progenitors through endothelial AT2R and cytoskeletal dysregulation. Nat Commun 2015 Jan 9;6:5914. doi: 10.1038/ncomms6914. PMID 25574809, NIHMS644197, PMC4293039

31. Privette Vinnedge LM, Benight NM, Wagh PK, Pease NA, Nashu MA, Serrano-Lopez J, Adams AK, Cancelas JA, Waltz SE, Wells SI. The DEK oncogene promotes cellular proliferation through paracrine Wnt signaling in Ron receptor-positive breast cancers. Oncogene 2015 Apr 30;34(18):2325-36. Epub Jun 23, 2014. doi: 10.1038/onc.2014.173. PMID 24954505, NIHMS594564, PMC4275425

32. Yamada Y, Cancelas JA, Rothenberg ME. Murine Models of Eosinophilic Leukemia: A Model of FIP1L1-PDGFRα Initiated Chronic Eosinophilic Leukemia/Systemic Mastocytosis. Chapter 26. In: Eosinophils. Methods and Protocols. Ed. GM Walsh. Humana Press, Springer, New York, 2014. P 326. (ISBN 978-1-4939-1015-1). Methods Mol Biol 2014;1178:309-320. PMID 24986627

33. Chang KH, Sengupta A, Nayak RC, Duran A, Lee SJ, Pratt RG, Wellendorf AM, Hill SE, Watkins M, Gonzalez-Nieto D, Aronow BJ, Starczynowski DT, Civitelli R, Diaz-Meco MT, Moscat J, Cancelas JA. p62 is required to retain short-term repopulating and myeloid progenitor cells through inhibition of IKK/NF-κB/Ccl4 signaling at the bone marrow macrophage-osteoblast niche. Cell Rep Dec 24 2014;9(6):2084-97. doi: 10.1016/j.celrep.2014.11.031. Epub Dec 18. PMID 25533346, NIHMSID 645385, PMC4277497

34. Fang J, Barker B, Bolanos L, Liu X, Jerez A, Makishima H, Christie S, Chen X, Rao DS, Grimes HL, Komurov K, Weirauch MT, Cancelas JA, Maciejewski JP, Starczynowski DT. Myeloid malignancies with chromosome 5q deletions acquire a dependency on an intrachromosomal NF-κB gene network. Cell Rep 2014 Sep 11;8(5):1328-38. doi: 10.1016/j.celrep.2014.07.062. Epub 2014 Sep 4. PMID 25199827, NIHMS62121, PMC4237069

35. Mock DM, Widness JA, Veng-Pedersen P, Strauss RG, Cancelas JA, Cohen RM, Epub Jun Lindsell CJ, Franco RS. Measurement of post-transfusion red cell survival with the biotin label. Transfusion Med Rev 2014 Jul;28(3):114-25. Epub Apr 5, 2014, http://dx.doi.org/10.1016/j.tmrv.2014.03.003. PMID 24969019. NIHMS585301, PMC4122272

36. Velu CS, Chaubey A, Phelan JD, Horman SR, Wunderlich M, Guzman ML, Jegga AG, Zeleznik-Le NJ, Chen J, Mulloy JC, Cancelas JA, Jordan CT, Aronow BJ, Marcucci G, Bhat B, Gebelein B, Grimes HL. Therapeutic antagonists of microRNAs deplete leukemia-initiating cell activity. J Clin Invest 2014 Jan 2;124(1):222-36. Epub Dec 16, 2013. PMID 24334453 PMC3871218

37. Tidwell T, Wechsler J, Nayak RC, Trump L, Salipante SJ, Cheng JC, Donadieu J, Glaubach T, Corey SJ, Grimes HL, Lutzko C, Cancelas JA, Horwitz MS. Neutropenia-associated ELANE mutations disrupting translation initiation produce novel neutrophil elastase isoforms. Blood 2014;123(4):562-569. Epub Nov 1, 2013. PMID 24184683 PMC3901069

38. Taniguchi Ishikawa E*, Chang KH*, Nayak R, Olsson HA, Ficker AM, Dunn SK, Madhu MN, Sengupta A, Whitsett JA, Grimes HL, Cancelas JA. Klf5 controls bone marrow homing of stem cells and progenitors through Rab5-mediated membrane β1/β2-integrin expression. Nat Commun 2013 Apr 3;4:art. No. 1660. doi: 10.1038/ncomms2645. (*equal contributing authors) PMID 23552075, NIHMS448853, PMC3627399

39. Raman R, Kumar RS, Hinge A, Kumar S, Nayak R, Xu J, Szczur K, Cancelas JA, Filippi MD. p190-B RhoGAP regulates the functional composition of the mesenchymal microenvironment. Leukemia Nov 2013;27(11):2209-19. Epub Apr 8 2013. PMID 23563238, NIHMS549952, PMC3919554.

40. Nayak RC, Chang K-H, Vaitinadin N-S, Cancelas JA. Rho GTPases control specific cytoskeleton dependent functions of hematopoietic stem cells. [Review] Immunol Rev 2013 Nov;256(1):255-268. PMID 24117826, NIHMS524728, PMC3830525.

41. Zhou X, Florian MC, Arumugam P, Chen X, Cancelas JA, Lang R, Malik P, Geiger H, Zheng Y. RhoA GTPase controls cytokinesis and programmed necrosis of hematopoietic progenitors. J Exp Med 2013 Oct 21; 210(11):2371-2385. Epub Oct 7 2013. PMID 24101377, PMC3804933.

42. Mayes DA, Rizvi TA, Titus-Mitchell H, Oberst R, Ciraolo GM, Vorhees CV, Robinson AP, Miller SD, Cancelas JA, Stemmer-Rachamimov AO, Ratner N. Nf1 loss and Ras hyperactivation in oligodendrocytes induce NOS-driven defects in myelin and vasculature. Cell Rep 2013 Sep 26;4(6):1197-212. Epub 2013 Sep 11 PMID 24035394, NIHMS567618, PMC3982616.

43. Guo F, Zhang S, Grogg M, Cancelas JA, Varney ME, Starczynowski DT, Du W, Yang J-Q, Liu W, Thomas G, Kozma S, Pang Q, Zheng Y. Mouse gene targeting reveals an essential role of mTOR in hematopoietic stem cell engraftment and hematopoiesis. Haematologica Sep 2013;98(9):1353-8. Epub May 28, 2013.PMID 23716557 PMC3762090.

Patents

Prolonged Storage of Red Blood Cells and Composition

Hess JR, Greenwalt TJ

Novel additive solutions that are useful for the storage of human red blood cells (RBCs) under refrigerated conditions. Also, a method of using the additive solutions in an appropriate volume to preserve RBCs at about 1 to 6°C for up to 10 weeks. The solutions and processes allow the viable storage of human RBCs for an extended period of time in a solution which is directly infusible in humans.

Issue Date: November 21, 2000 US Patent # 6150085

Preservation and Shelf Life Extension of Blood Cells

Hess JR, Greenwalt TJ

Preservative solutions that extend the useful shelf-life of red cells from the current 6 to 12 weeks. This intellectual property is shared by the US Army and the University of Cincinnati. Implementation is expected to save 600,000 units from outdating annually, to reduce seasonal shortages of blood, and to make autologous donor programs more efficient.

Issue Date: November 20, 2001 US Patent # 6447987

Compositions Substantially Free of Sodium Chloride and Methods For The Storage of Red Blood Cells

Hess JR, Greenwalt TJ

Composition of a solution for storage of red blood cells consisting essentially of: adenine; dextrose; a non-metabolizable membrane-protectant sugar; and a specifically defined pH buffering system. Improved methods for preserving red blood cells and methods for increasing the viability, membrane retention, and recoverability while suppressing apoptosis, hemolysis, and post-reinfusion clearance of stored red blood cells which utilize the novel compositions.

Issue Date: April 29, 2014 US Patent # 8709707